Corneal abrasions are one of the most common anesthesia-related adverse events. There is limited evidence about the effect of virtual education on anesthesia providers’ knowledge regarding corneal abrasion prevention. The purpose of this study project was to assess if a web-based educational module effectively improved anesthesia providers’ awareness of evidence-based preventative measures of corneal abrasions. This was a quasi-experimental pretest-posttest study design. All participants completed a questionnaire regarding their knowledge of corneal abrasion prevention before and after the educational module. A student T-test was used to compare the differences in pre- and post-education knowledge scores. There were 55 participants in the study. Among them, 23.6% were anesthesiologists and 76.4% were certified registered nurse anesthetists. There was a significant pretest-posttest difference in the total knowledge score (6.49±1.23 vs. 8.24± 1.08, p< .001). The average number of correct answers increased from 6.49 ± 1.23 to 8.24 ± 1.108 (t = 9.94, p < .001) Overall, the web-based educational module was effective in enhancing anesthesia providers’ knowledge in preventing surgical-induced corneal abrasions. The finding may add evidence for developing effective guidelines and standardizing provider education in corneal abrasion prevention.